To further develop an economical fermentation and recycling efficiently by-products using strain S47, fed-batch fermentation was also conducted. lipolytica S47, which is capable of expressing SIase and transforming monosaccharides to lipids as shown. In this study, pretreated cane molasses (PCM) was used as the only carbon source, and corn steep liquor (CSL) was selected as substrate to replace yeast extract for the recombinant Y. On the other hand, the synthesis of isomaltulose from cane molasses can greatly improve the value of cane molasses. The high sucrose content in cane molasses makes it a promising substrate for isomaltulose production. However, the strain used in this study can just utilize monosaccharides, leaving sucrose not hydrolyzed for no sucrase generating. In traditional cane molasses fermentations using sucrose as the carbon source, sucrose was hydrolyzed into monosaccharides and utilized by microorganisms. Different high value-added metabolites have been harvested via microbial fermentation from molasses, such as astaxanthin, 5-hydroxymethylfurfural, ethanol, organic acids, and enzymes. Given that available ingredients enrich in cane molasses, nowadays, it is increasingly utilized as an alternative feedstock for microbial fermentation after a pretreatment or as the raw material. However, livestock frequently suffers from some nervous symptoms and blindness caused by molasses toxicity.
Cane molasses can be used as an available source of quick energy for animal feed or feed supplement. The molasses production in China is around 400 million tons per year, yet, large volumes of waste molasses are simply discharged, contributing to severe environmental pollution. Sugarcane grows worldwide and mainly distributes in Brazil, India, and China. Cane molasses is one of the main by-products of sugar refineries, which contains saccharides (primarily sucrose, glucose and fructose) and a small amount of nitrogenous compounds, vitamins, and trace metal elements as well as colloids.
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